Extended Data Fig. 3: The maternal-to-zygote transition is disorganized in human arrested embryos.
a. Venn plots showing the overlap between major ZGA genes and up-regulated genes in human arrested zygotes to 4-cell embryos. b. Radar chart showing the average expression levels of representative major ZGA genes in human TUBB8mutant arrested zygotes from Sha et al.4 and human normal zygotes from Yan et al.19 from 2, 3 biologically independent samples; integration of all samples. c. Expression patterns and GO terms of each cluster of up-regulated major ZGA genes in human arrested embryos. Left, heatmap showing k-means clustering (n = 4) of major ZGA genes that are commonly up-regulated in human arrested zygotes to 4-cell embryos. The color key from blue to red indicates the expression level from low to high. Middle, line plots showing the average expression patterns of genes in each cluster. Right, GO terms of genes in each cluster. In a, c, hypergeometric test without adjustment. d-f. Line plots showing the expression levels of d. SVA, e. representative TEs and f. M-decay genes in human normal and arrested embryos at each stage. TEs, transposable elements. CPM, count-per-million. The number of biologically independent samples is 9, 10, 10, 11, 10, 9 for human normal MII to morula; 18, 13, 8, 7 for human arrested zygote to 8-cell embryos. g, h. g. RT-qPCR results showing the relative expression levels of M-decay gene EIF3K in human normal (3PN) and arrested zygotes or 2-cell embryos (2PN). Each dot represents an embryo. Data are from 4, 4, 6, 3, 5 biologically independent samples. h. RT-qPCR results showing the relative expression levels of M-decay genes PABPC1L, TLE6, ZP2 in human normal (3PN) and arrested zygotes (2PN). Data are from 4, 4, 6 biologically independent samples. In c-h, error bars represent the mean ± SEM. In g, h, two-sided Mann-Whitney U test.
