Extended Data Fig. 6: YY1 promotes human zygotic genome activation.
a. Regulatory network of key transcription factors (TFs) that are shared or unique among human arrested zygotes to 4-cell embryos predicted by SCENIC analysis. b. Bar graph showing the number of target genes that are regulated by TFs in human arrested zygotes to 4-cell embryos predicted by SCENIC analysis. Bars are colored according to developmental stage; integration of all samples. c. Line plots showing the average expression levels of KLF4 and ZFP42 in human normal MII to morula and arrested embryos at each stage. The number of biologically independent samples is 9, 10, 10, 11, 10, 9 for human normal MII to morula; 18, 13, 8, 7 for human arrested zygote to 8-cell embryos. d. Venn plot showing the overlap between major ZGA genes and the target genes of KLF4 or ZFP42 identified from public ChIP-seq data in human embryonic stem cells (hESCs)33,34. Hypergeometric test without adjustment. e. Venn plot showing the overlap among target genes that are regulated by YY1, KLF4 or ZFP42 identified from public ChIP-seq datasets in hESCs or HEK 293T cells32,33,34. f. Boxplots showing the average expression levels of major ZGA genes in human 2PN (from Yan et al.19, n = 16) and 3PN 8-cell embryos (n = 16). Data are from 3, 4 biologically independent samples. Each box represents the median, 25% and 75% quartiles; whiskers indicate 1.5 times the interquartile range. Unpaired two-sided Student’s t-test. g. Bar plot showing the expression levels of YY1 among YY1 KD and control human 8-cell embryos (developed from human 3PN zygotes). Each point represents an embryo. Data are from 3, 3, 2 biologically independent samples. In c, g, error bars represent mean ± SEM. h. Genome browser views showing YY1 signals from public ChIP-seq data in hESCs32.
