Extended Data Fig. 8: Aberrant reprogramming of DNA methylation and chromatin accessibility link with cytoskeleton defects in human arrested embryos.
a. Venn plot showing the overlap of genes associated with arrested-specific hypo-DMRs (promoter) among human arrested zygotes to 8-cell embryos. b. Differences in the DNA methylation level of autophagy and RNA splicing genes (promoter) between human arrested and normal embryos at each stage. Data are from biologically independent samples as indicated in Fig. 1f; integration of all samples. c. Genome browser views showing the DNA methylation levels of cytoskeletal and mitotic related genes in human normal and arrested embryos at each stage. The expression levels of genes are shown (right). The WCG sites detected with DNA methylation lower than 1/10 of the data range (but are detected) are shown as black bars. d. GO terms of genes associated with arrested-specific NDRs (see details in the Method section) and normal-specific NDRs (promoter) in human arrested embryos at each stage. Bars are colored according to developmental stage. Genes in GO terms that are consistent with gene expression levels are colored in red. Hypergeometric test without adjustment.
