Extended Data Fig. 1: Quality control and initial processing of mouse scRNA-seq data.

a,b Quality control metrics of data, including number of genes detected (‘nFeature_RNA’), number of unique molecular identifiers detected (‘nCount_RNA’), and percent mitochondrial reads (‘% mito GE) before (a) and after (b) processing data. c-e UMAP of total murine epithelial cells sequenced post-QC, coloured according to mouse identity (c), cell type annotation (d), or cell cycle phase (e). f Frequency of epithelial cells of indicated subtype by segment. QC, quality control, mito, mitochondrial; GE, gene expression; ISC, intestinal stem cell; TA, transit amplifying; G1, growth 1; G2M, growth 2 mitosis; S, synthesis.