Extended Data Fig. 9: The desaturase SCD1 contributes to blastocyst implantation, and the desaturases for the PUFA and MUFA synthesis mediate apical-basolateral protein expression in TSCs and ESCs.
From: Low-input lipidomics reveals lipid metabolism remodelling during early mammalian embryo development
(a) A table showing the number of transfered embryos and implanted embryos with and without knocking down Scd1. (b) Immunofluorescence of E-cadherin in ESCs treated with DMSO, CAY10566(1uM), SC-26196(1uM), sesamin(1uM). Arrows indicate E-cadherin localization. (c) Immunofluorescence of E-cadherin in TSCs treated with DMSO, CAY10566(1uM), SC-26196(1uM), sesamin(1uM). Arrows indicate E-cadherin localization. (d, e) Immunofluorescence of K8 and K18 in TSCs treated with DMSO, CAY10566(1uM), SC-26196(1uM), sesamin(1uM). Arrows indicate K8 and K18 localization. (f, g) Immunofluorescence of phosphorylated-ezrin(Thr567) in ESCs (f) and TSCs (g), respectively, treated with DMSO, CAY10566(1uM), SC-26196(1uM), sesamin(1uM). Arrows indicate Phospho-ezrin localization. (b-g) The images shown are representative of three independent experiments; scale bar, 30uM.
