Extended Data Fig. 10: Desaturases for the PUFA and MUFA synthesis are required for the generation of EPS-blastoids.
From: Low-input lipidomics reveals lipid metabolism remodelling during early mammalian embryo development
(a) Representative phase-contrast images of EPSC aggregates at the indicated time to show the EPS-blastoid formation; the images shown are representative of three independent experiments. (b) Phase-contrast images of blastoid formation in the DMSO-, SC-26196 or sesamin-treated conditions; the images shown are representative of two independent experiments. (c) Quantification of blastoid formation efficiency in the DMSO-, SC-26196 and sesamin-treated conditions. Data are from two independent experiments. (d) Immunofluorescence of E-cadherin and K18 localization in the blastoids treated with DMSO-, SC-26196 and sesamin; the images shown are representative of two independent experiments. (e) A schematic to show apical-basal polarity establishment, lateral expansion of apical domain protein phospho-ezrin and cytoskeleton proteins F-actin and Keratin, and the zippering process to seal the blastocyst embryo along the cell-cell junction during the eight-cell-to-blastocyst embryo development. The desaturase SCD1-mediated lipid unsaturation, membrane fluidity and cytoskeleton dynamics are required for these processes to take place in order to support successful blastocyst implantation in the uterus.
