Extended Data Fig. 8: DSB end-joining induces aberrant C > G transversion.
From: C-to-G editing generates double-strand breaks causing deletion, transversion and translocation
a, C > G purity at three loci in CasRx-knockdown HEK293T cells. Each dot indicates average C > G purity of each positions from three biological replicates. The RFWD3 mRNA levels are shown on the left (mean ± SD of 3 biological replicates). b, Genotoxicity of REV1-deficient HEK293T cells after UV treatment. Mean ± SD of 3 replicates is shown. Knockout of REV1 was validated by Western blot. Representative blot is shown from 2 replicates. c, C > G purity after CGBE editing is plotted for each locus. Mean ± SD of 3 replicates is shown. Expression levels of CGBE1 in the indicated cells are shown by a representative Western blot from 2 replicates. A 3-fold serial dilution of protein samples was applied in Western blot. d, C > G purity is plotted for 3 loci in the Rev1-/-, Rev7-/-, Rev3l-/- and parental CH12F3 cell line. Mean ± SD of 3 independent replicates is shown. e, Expression levels of CGBE1 in the indicated cells are shown by a representative Western blot. A 3-fold serial dilution of protein samples was applied in Western blot. f, C > G purity after CGBE editing is plotted for each locus. Mean ± SD of 3 replicates is shown. g, Fold change of C > G purity in XRCC4 deficiency vs. wild-type is plotted against that of deletion frequency at 11 sgRNA loci. Linear regression analysis was performed and Pearson’s r is shown. The significance of regression model was calculated by the F-test. h, A working model of C > G transversion. For Panels a, c and f, two-tailed Student’s paired t-test was applied. For Panel b, two-tailed Student’s unpaired t-test was applied. For boxplots in a and c, centre line indicates the median; box limits represent the upper and lower quartiles, and the whiskers represent minimum to maximum values. Source numerical data and unprocessed blots are available in source data.
