Extended Data Fig. 10: High-fidelity Cas or optimized deaminase module cannot reduce CGBE-initiated deletion and GFP gating strategy. | Nature Cell Biology

Extended Data Fig. 10: High-fidelity Cas or optimized deaminase module cannot reduce CGBE-initiated deletion and GFP gating strategy.

From: C-to-G editing generates double-strand breaks causing deletion, transversion and translocation

Extended Data Fig. 10: High-fidelity Cas or optimized deaminase module cannot reduce CGBE-initiated deletion and GFP gating strategy.

a, CGBE1-derived tools with modified Cas/deaminase modules are shown on the top. Substitution frequency, C > G purity, deletion frequency, and insertion frequency were shown side-by-side between CGBE1 and optimized tool at 4 tested loci. Data from two independent replicates are shown. Source numerical data are available in source data. b, GFP gating strategy. In the transfection efficiency control assay, cells co-transfected with a GFP-expressing plasmid were gated on FSC and SSC for live cells. Live cells were further gated on FITC intensity. Non-transfected cells were assayed as a negative control.

Source data

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