Extended Data Fig. 3: Insertion profiles generated by BEs.
From: C-to-G editing generates double-strand breaks causing deletion, transversion and translocation
a, Insertion profiles are shown at the indicated loci after cytidine editing. 1bp-insertion are shown at the top and >1bp-insertion are shown at the bottom for each locus. In the profile, the inserted position was counted and the frequency is plotted along the nucleotides. b, Insertion subgroups. 1-bp insertions at a mononucleotide track (“Mono”), editing sites (“Edit”), or the Cas9-nicking site (“Nick”), and >1 bp insertions with duplication sequences are shown at the top. Example insertions are labelled along the DNA sequences. The count number of a particular insertion is shown in different colors from the total 1.9 million sequencing reads. c, Substitution, deletion and >1 bp insertion frequencies are summarized for the indicated tools at 4 tested loci from three biological replicates. For the Cas9 module, dCas9 (d) or nCas9 (n) was tested; for the AID/APOBEC module, catalytically active (+) or dead (-) module was tested. Two-tailed Student’s paired t-test was applied. For boxplot, centre line indicates the median; box limits represent the upper and lower quartiles, and the whiskers represent minimum to maximum values. Source numerical data are available in source data.
