Extended Data Fig. 2: Characterization of TFAM-deficient U2OS cells derived using CRISPR. | Nature Cell Biology

Extended Data Fig. 2: Characterization of TFAM-deficient U2OS cells derived using CRISPR.

From: Mitochondrial DNA replication stress triggers a pro-inflammatory endosomal pathway of nucleoid disposal

Extended Data Fig. 2

a) Sequencing of U2OS TFAM-deficient clones, confirming insertion and frameshifting in exon 1 (top) relative to unedited U2OS cells (bottom). Similar results were obtained for both clones. b) Western blotting of TFAM and β actin (loading control) in two TFAM-deficient CRISPR clones (TFD-1 and TFD-2) as well as the parental U2OS cells. c) Quantification of B. Dots represent replicates of independent experiments (N = 3). d) mtDNA abundance (relative mtDNA copy number) analyzed by qPCR with D-loop and ND1 primers, normalized to nuclear 18 S. Dots represent replicates (N = 3). All data are reported as mean ± SEM. Source numerical data and unprocessed blots are available in source data.

Source data

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