Extended Data Fig. 7: HSV1 does not trigger re-localization of enlarged nucleoids into autophagosomes.
a) Spinning disk imaging of LC3, TFAM, and HSP60 immunofluorescence in a U2OS cell 8 hours after infection with HSV1-GFP. Larger scale bar = 10 µm and inset scale bars = 1 µm. This experiment was performed three times with similar results. b) The number of non-mitochondrial TFAM puncta that overlapped with LC3 was scored. Mock infected cells were compared to cells infected for 8 hours (p = 0.3135). Number of cells: N = 31 for mock, N = 33 for 4 hr, N = 31 for 6 hr, N = 32 for 8 and 10 hr, and data were pooled from three independent experiments. The same dataset was used to generate both graphs. c) Spinning disk imaging of LC3 immunofluorescence in U2OS cells infected with HSV1-GFP and fixed at the indicated timepoints after infection. Scale bars = 10 µm. This experiment was performed three times with similar results. d) The number of LC3 puncta per cell was scored and compared between mock and infected cells (p = 0.0047 for 4 hr, p < 0.0001 for 6, 8, and 10 hr). e) The mean fluorescence intensity within LC3 puncta was measured (see Methods) and compared between mock and infected cells (p = 0.2544 for 4 hr, p < 0.0001 for 6, 8, and 10 hr). For panels D and E, N = 12 cells per condition, and data were quantified from one representative experiment of three. For all plots, line represents mean, and all graphs represent mean ± SEM. All differences were compared using unpaired, two-tailed student’s t test. Source numerical data are available in source data.
