Extended Data Fig. 8: ECSIT deficiency impairs CD8+ T cells antitumour function through cell-intrinsic mechanisms. | Nature Cell Biology

Extended Data Fig. 8: ECSIT deficiency impairs CD8+ T cells antitumour function through cell-intrinsic mechanisms.

From: ECSIT facilitates memory CD8+ T cell development by mediating fumarate synthesis during viral infection and tumorigenesis

Extended Data Fig. 8: ECSIT deficiency impairs CD8+ T cells antitumour function through cell-intrinsic mechanisms.

(a) Schematic of tumour model. WT recipient mice were implanted subcutaneously with B16F10-OVA cells, and then Ecsitfl/fl OT-1 or Ecsitfl/fl Rosa26CreERT2 OT-1 naive CD8+ T cells were transferred into recipient mice 1 day later. Tamoxifen was injected intraperitoneally 5 times from day10 to day15. The growth of B16F10-OVA melanomas and the survival of mice were assessed. And transferred OT-1 cells were analysed on day 20. (b) Schematic of tumour model with adaptive co-transplantation. CD45.1 recipient mice were implanted subcutaneously with B16F10-OVA cells, and then Ecsitfl/+ CD45.1.2 OT-1 and Ecsitfl/fl Rosa26CreERT2 CD45.2 OT-1 naive CD8+ T cells were mixed and transferred into recipient mice 1 day later. Tamoxifen was injected intraperitoneally 5 times from day10 to day15. Transferred OT-1 cells were analysed on day 20.

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