Extended Data Fig. 9: ECSIT affects chromatin accessibility of TME CD8+ T cells and promotes transcription regulation of Tcf7 in chronic tumour stimulation. | Nature Cell Biology

Extended Data Fig. 9: ECSIT affects chromatin accessibility of TME CD8+ T cells and promotes transcription regulation of Tcf7 in chronic tumour stimulation.

From: ECSIT facilitates memory CD8+ T cell development by mediating fumarate synthesis during viral infection and tumorigenesis

Extended Data Fig. 9: ECSIT affects chromatin accessibility of TME CD8+ T cells and promotes transcription regulation of Tcf7 in chronic tumour stimulation.

(a) Flow cytometry gating strategy of sorting Ecsitfl/+ CD45.1.2 OT-1 and Ecsitfl/fl Rosa26CreERT2 CD45.2 OT-1 cells (memory) from B16F10-OVA tumour-bearing CD45.1 recipient mice for RNA-seq and ATAC-seq. (b) GO and KEGG analysis of differentially accessible chromatin region genes. (c) Genome track view of the Ccr7 and Slamf6 locus showing ATAC-seq and Tcf7 CUT&RUN peaks (GSE139056). Tcf7 predictive binding sites (red shadows) are listed. One-side Fisher’s exact test for (b).

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