Extended Data Fig. 1: ECSIT is upregulated in memory CD8⁺ T cells, and promotes memory CD8⁺ T cells generation in a cell-intrinsic manner under the homeostasis.

(a) Representative flow cytometry plots of Ecsit–EGFP expression in immune cells in Fig. 1d. (b) Representative flow cytometry plots of Ecsit–EGFP expression in T_N, T_CM, and T_EM of CD8⁺ T cells in Fig. 1e. (c) Representative flow cytometry plots of Ecsit–EGFP expression in MPEC and SLEC of CD8⁺ T cells in Fig. 1f. (d) Representative flow cytometry plots of Ecsit–EGFP expression in T_EM and T_CM of CD8⁺ T cells in Fig. 1g. (e) Representative flow cytometry plots of Ecsit–EGFP expression in T_SCMP and T_EXH of CD8⁺ T cells in Fig. 1h. (f) CD45.1 mice bone marrows were mixed equally with Ecsit^fl/fl or Ecsit^fl/fl dLck-Cre (CD45.2) mice bone marrows then injected intravenously into irradiated Rag1^−/− mice. 8 weeks later, draining lymph nodes were collected and analysed by flow cytometry for CD4⁺ T cells, CD8⁺ T cells, and CD8⁺ T_N, T_M cells. Data are pooled from three independent experiments and n = 6 for (f). Error bars show mean ± sem. Two-tailed unpaired student’s t-test for (f).

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