Fig. 1: ERCC6/CSB mediates cellular tolerance of DPCs.
From: Transcription-coupled repair of DNA–protein cross-links depends on CSA and CSB
a, Schematic of formaldehyde (FA) and 5-aza-dC CRISPRi screens compared to non-treated conditions (NT) in K562 cells. b, Rank plot showing normalized Z-scores (NormZ) scores from DrugZ analysis and selected hits from the formaldehyde CRISPRi screen in a. c, The same as for b but for the 5-aza-dC CRISPRi screen. d,e, Clonogenic survival assays in WT, CSB−/− and XPA−/− HAP1 cells treated with formaldehyde (d) or 5-aza-dC (e). Error bars ± s.e.m., n = 4 replicates. f, Doxycycline (dox)-inducible expression of CSB or CSBK538R in CSB−/− TET3G HAP1 cells; representative of three independent experiments. g–i, Clonogenic survival assays in WT or CSB−/− TET3G cells with doxycycline-induced expression of CSB, CSBK538R or the empty vector (EV) treated with formaldehyde (g), 5-aza-dC (h) or illudin S (i). Symbols and error bars denote mean ± s.e.m., n = 3 replicates. j–l, Alamar blue cell viability assays in the indicated RPE1 cell lines treated with formaldehyde (j), 5-aza-dC (k) or illudin S (l). Symbols and error bars denote mean ± s.d., n = 3 replicates. Source numerical data and unprocessed blots are available in Source data.
