Extended Data Fig. 2: CSB acts in parallel to known DPC repair pathways in promoting DPC tolerance.
From: Transcription-coupled repair of DNA–protein cross-links depends on CSA and CSB
(a) Western blot for CSB and SPRTN in WT, CSB−/−, SPRTN-ΔC and SPRTN-ΔC/CSB−/− RPE1 cell line clones; data are representative of 2 independent experiments. (b) Colony formation assay in untreated cell lines from (a). (c) siRNA-mediated depletion of CSB in WT and SPRTN-ΔC RPE1 cells. (d) siRNA-mediated depletion of SPRTN in WT and CSB−/− RPE1 cells. (e-f) Depletion of RNF4 by siRNA transfection in WT and CSB−/− RPE1 (e) or HAP1 (f) cells. (c-f) data are representative of 3 independent experiments. (a) and (c): the monoubiquitylated form of SPRTN is denoted by an asterisk (*) and the truncated SPRTN-ΔC protein product is denoted by two asterisks (**). Unprocessed blots are available in source data.
