Extended Data Fig. 3: DPC repair is more efficient across actively transcribed genes.
From: Transcription-coupled DNA–protein crosslink repair by CSB and CRL4CSA-mediated degradation
a: DPC repair % in expressed bins was plotted against their expression level. Spearman correlation between DPC repair and transcription levels was 0.61997. b: A graphic representation of the RT-qPCR primers used in the DPC removal assay along the CRIM1 gene. c: Relative DPC recovery in untreated (NT) or after the indicated times after a 30 min 1 mM FA pulse using K-SDS precipitation followed by qPCR of genomic DNA in the CRIM1 gene and CRIM1 intergenic region as well a silent genomic region on chromosome Y. Values were normalized to 0 hr conditions for each primer set at 1 and represent mean with S.E.M. from 4 independent experiments. d: Relative DPC recovery at 4 hr after a 30 min 1 mM FA pulse using K-SDS precipitation followed by qPCR of genomic DNA in the CRIM1 gene and CRIM1 intergenic region. Values were normalized to 0 hr conditions (not shown) for each primer set at 1 and represent mean with S.E.M. from 4 independent experiments. Source numerical data are available in source data.
