Fig. 1: Pooled protein tagging for the generation of vpCell pools.
From: Pooled multicolour tagging for visualizing subcellular protein dynamics
a, Overview of the visual proteomics vpCell approach. b, Schematic of the pooled intron-tagging approach. c, Abundance of sgRNAs in the GFP-positive cell pool after protein tagging. d, Comparison of tagging efficiencies when targeting different genes with high-efficiency sgRNAs or other sgRNAs from the library. Data are shown as mean ± s.d.; n = 3 biologically independent experiments. e, Expression level of proteins targeted by high-efficiency sgRNAs and proteins targeted by all sgRNAs in the library. Boxes represent 25th, 50th and 75th percentiles, and whiskers represent 10th and 90th percentiles. P value < 10−200, two-sided Student’s t-test; n = 58,472 sgRNAs with available expression levels of target proteins, examined over one pooled protein tagging experiment. f, Relative position of the tag sites in the protein of high-efficiency sgRNAs and all sgRNAs in the library.
