Fig. 2: Generation of multicolour cell pools.
From: Pooled multicolour tagging for visualizing subcellular protein dynamics
a, Schematic of the generation of multicolour cell pools by performing a second round of intron tagging using a sgRNA library targeting frame 1 introns and a matching DNA donor followed by lentiviral transduction with fluorescent markers. b, Visual barcodes. Fluorescence microscopy images of HAP1 cells transduced with constructs expressing BFP localizing to different subcellular compartments. Scale bars, 25 µm. NLS, nuclear localization signal; NES, nuclear export signal. c, Stitched image from multiple FOVs of a multicolour pool, 2 days after seeding. Representative of n = 3 tagging experiments. Scale bar, 100 μm. d, Examples of clonal cell lines isolated from the multicolour pool. Scale bars, 25 μm.
