Extended Data Fig. 3: Generating a multicolour intron-tagged cell pool.
From: Pooled multicolour tagging for visualizing subcellular protein dynamics
a, Flow cytometry after the second round of intron tagging of HEK293T cells that were transfected with frame1 intron tagging plasmids after being transduced with a frame 1 genome-wide intron targeting sgRNA library, compared to HEK293T cells that were transfected with frame 1 intron tagging plasmids and a plasmid expressing a sgRNA targeting intron 3 of MTHFD2, compared to HEK293T cells transfected with frame 1 intron tagging plasmids, without any frame 1 intron-targeting sgRNA present, compared to HEK293T that were not transfected after being transduced with a frame 1 genome-wide intron targeting sgRNA library. b, Fluorescence microscopy images of HAP1 cells transduced with miRFP670-miRFP670nano localizing to the nucleus and mAmetrine localizing to the cell membrane. Scale bars: 25 µm.
