Extended Data Fig. 6: Symmetric cell divisions in late organoids.
From: Cerebral organoids display dynamic clonal growth and tunable tissue replenishment
a, Representative example of an organoid at day 42 with lineage tracing virus (GFP, green) from the same experiment as used for scRNA-seq. b, FACS plots of sorted cells for scRNA-seq analysis per organoid. (n = 2 organoids as biological replicates from 1 differentiation experiment). c-e, UMAPs of day 18 organoid cells in scRNA-seq indicating cell types (b), select markers for cell type annotation (c) and cell classifications in S- plus A- and N-cells (d). Ips, intermediate progenitors. (n = 2 organoids as biological replicates from 1 differentiation experiment). f, g, Lineage size rank plots for day 18 organoid 1 (f) and 2 (g). h, i, UMAP of individual organoids at day 18 (h) and day 42 (i). j, k, Immunofluorescent characterization of cerebral organoids at day 40 for nuclei (DAPI), incorporated BrdU (1 h pulse 24 h prior to fixation), dividing cells (pVIM, Ser 82) and neural stem cells (SOX2). Example 2 (j) and 3 (k). See Fig. 3g, h, for overview and example 1. l, Live imaging example 2. See Fig. 3i–m for overview and quantification.
