Extended Data Fig. 9: Replenishment in RFP-WT with GFP-KO chimeric organoids.
From: Cerebral organoids display dynamic clonal growth and tunable tissue replenishment
a-d, Immunofluorescent analysis of day 42 organoids chimeric for RFP-WT and GFP-WT (a), GFP-KO-TP53 (b), GFP-KO-ASPM (c) and GFP-KO-PAX6 (d). Labelling of fluorophores and neuronal marker MAP2 as indicated. e-h, Whole organoid lineage tracing of chimeric organoids containing 50% RFP-WT and 50% GFP-WT (e), GFP-KO-TP53 (f), GFP-KO ASPM (g) and GFP-KO-PAX6 (h) cells. (n = individual lineages from 3 organoids as biological replicates from 1 differentiation experiment at each time point). i, Optimal transport comparison of lineage populations of RFP-WT to chemically grown intra-organoid GFP-WT or selected GFP-KO cells over time. Colour code for time indicated at the right, thickness of curves indicates number of lineages of certain sizes. (n = individual lineages from 3 organoids as biological replicates from 1 differentiation experiment at each time point).
