Extended Data Fig. 4: Timing of neuroepithelium formation at day 11 in cerebral organoids and immunofluorescent characterization of organoid cell composition over time.
From: Cerebral organoids display dynamic clonal growth and tunable tissue replenishment
a, Immunofluorescent characterization of organoids at indicated timepoints using apical (aPKC) and stem cell (Nestin) markers. b, Immunofluorescent characterization of organoids at indicated timepoints using adherens junction markers (b-catenin, n-cadherin). c, Immunofluorescent characterization of cerebral organoids at indicated timepoints on the left and markers for nuclei (DAPI), neural stem cells (Nestin, SOX2) and neurons (TUJ1, DCX, MAP2) indicated on the top. Note that fibres in organoids display autofluorescence. Adjacent cryosection per timepoints were used.
