Extended Data Fig. 6: The role of FBL on rRNA transcription and rRNA 2’-O-Me modification in AML cells.
a, Immunoblot analysis of H2AQ104me modification using anti-H2AQ104me in FBL-KD HL-60 cells rescued by different FBL mutants as indicated. b and c, Representative images (b) and quantitative summary (c) of nascent rRNA in FBL-KD HL-60 cells expressing WT FBL or the indicated mutants. n = 46 (shCT + EV and shFBL + MD-GAR), 47 (shFBL + EV and shFBL + FBL), 44 (shFBL + 4KR/4KQ), 40 (shFBL + MUT-E), 51 (shFBL + MD), 50 (shFBL + H2B-MD), 39 (shFBL + RGG-MD), 54 (shFBL + GAR) cells from 2 independent replicates. Kruskal-Wallis test. d and e, Quantification of total 2’-O-Me sites (Methscore >0.8) in different rRNA species (d) or of four different nucleotides (adenosine, uridine, guanosine and cytidine). f-h, Methscore values for significant downregulated 2’-O-Me sites in 28 S (f), 18 S (g) and 5.8 S (h) detected by RiboMeth-seq in FBL-KD HL-60 cells, compared to the control group. n = 3 biological replicates. Lines indicate the sites subsequently validated by RTL-P. i, RTL-P assays were conducted to confirm the downregulation of 2’-O-Me sites in 28 S and 5.8 S and 18 S by FBL deficiency as indicated in f-h. n = 3 biological replicates. One-way ANOVA with Dunnett’s test. Mean ± SEM (c-i).
