Fig. 6: SCD inhibitors sensitize to ferroptosis in translationally relevant settings.

a,b, Reciprocal effect of SCD1 inhibition and FADS2 inhibition on TGFβ-induced ferroptosis sensitivity in A549 and H358 lung carcinoma cells. Relative cell viability of A549 (a) and H358 (b) cells that were stimulated with 5 ng ml−1 TGFβ for 4 and 9 days, respectively, then pretreated with 5 µM MF438 (left) or 10 µM sc-26196 (right) for 24 h, and exposed to the indicated concentrations of ML210 for 48 h (H358) or 72 h (A549). Data are presented as mean ± s.e.m. from n = 3 independent experiments; ordinary two-way ANOVA. c, Relative cell viability of control and erlotinib-resistant (TR) H358 cells treated with 5 µM MF438 (left) or 10 µM sc-26196 (right) for 24 h, followed by ML210 treatment for 72 h. Data are mean ± s.e.m. from n = 3 independent experiments; ordinary two-way ANOVA. NS, not significant.