Extended Data Fig. 1: Microtubule morphology in 2D and 3D collagen hydrogel models.
From: Compression-dependent microtubule reinforcement enables cells to navigate confined environments
(a) 1205Lu melanoma cell CRISPR-edited to express meGFP-α-tubulin migrating in 2D. LUT inverted to enhance visibility of microtubule organization. (b) 1205Lu-meGFP-α-tubulin (orange heatmap) expressing cell migrating through a CNA35-Halo-JF549 labelled collagen I hydrogel (2.5 mg/mL; cyan heatmap) imaged by spinning disc confocal microscopy (SDCM). Visible distention of the cell body and nucleus can be observed where microtubules are seen to outline. (c) 1205Lu melanoma cell invading through a collagen hydrogel (DQ Collagen; white) labelled for F-actin (phalloidin, magenta) and nucleus (DAPI, cyan). Collagen aligns at cell-matrix attachment points (white arrowheads). The nucleus deforms as the cell invades (yellow arrowheads).
