Extended Data Fig. 9: LTβ-mediated signaling is essential to bone metastasis progression.
From: Lymphotoxin-β promotes breast cancer bone metastasis colonization and osteolytic outgrowth
a, SCP28 cells were IC injected into 6-week-old female nude mice. Mice were treated with LTβR-Ig recombinant protein three days later and continued until the experimental endpoint. b, Bone metastasis burden was monitored by BLI imaging from experiment in (a). n = 9 mice for IgG treated group, n = 12 for LTβR-Ig treated group. c, Kaplan-Meier BMFS curve of mice bearing bone metastasis in the experiment performed in (a). Group sizes as in (b).d, Representative BLI, μCT, H&E staining, and TRAP staining images of bone metastasis from the experiment in (a). Scale bar = 100 μm. BLI scale: x107 p/sec/cm2/sr. e, PD2R-LTβ cells were IC injected into 6-week-old female NSG mice. Mice were treated with LTβR-Ig recombinant protein three days later and continued until the experimental endpoint. f, Bone metastasis burden was monitored by BLI imaging from experiment in (e). n = 9 mice per each group. g, Kaplan-Meier BMFS curve of mice bearing bone metastasis in the experiment performed in (e). Group sizes as in (f). h, Representative BLI, μCT, H&E staining, and TRAP staining images of bone metastasis from experiment in (e). Scale bar = 100 μm. BLI scale: x107 p/sec/cm2/sr. Arrows indicate the osteolytic bone lesion areas (d,h). B, bone tissue area; T, tumor area (d,h). Data presented as mean ± SEM (b,f). The P values were determined by two-way repeated measures ANOVA (b,f) or log-rank test (c,g).
