Extended Data Fig. 10: LTβ promotes bone metastatic colonization in humanized mouse model.
From: Lymphotoxin-β promotes breast cancer bone metastasis colonization and osteolytic outgrowth
a, Humanized mouse model: human HSC cells were inoculated into lethally irradiated NSG mice to reconstitute their hematopoietic system. Peripheral blood from these mice was FACS analyzed for the presence of human CD45+ cells. Representative FACS-plot from this experiment was shown. b, Quantification of the percentage of human CD45+ cells from control NSG mice and from humanized mice. n = 3 mice for control group and n = 6 mice for humanized mouse group. c, GFP-labeled SCP28 cells with Vector control or LTβ OE were IC injected into humanized NSG mice. Mice were sacrificed 5 days later to collect hindlimbs for IF staining. Samples were counter-stained with DAPI. Scale bar = 100 μm. d, Quantification of GFP+ cells per field from experiment in (c). n = 8 fields per group. e, The α-LTβ antibody for IHC staining in Fig. 8f was verified by detecting LTβ expression in lymph node from patients with breast cancer. Rabbit IgG was utilized as a negative control. Data presented as mean ± SEM (b,d). P values were determined by two-tailed unpaired t-test (b,d).
