Extended Data Fig. 4: Ace2 and Muc1 RUSH data; Crb3 RUSH in MDCK cells.
From: A size filter at the Golgi regulates apical membrane protein sorting
(a) Related to Fig. 5: Aggregated Golgi depletion time data for Ace2 and Ace2-FKBP in polarized EpH4 monolayers, in the presence of SNAP-FRB ± CID. Each point is a cell, and each line joins paired measurements within the same cell. Datapoints are colored by experiment, with diamonds representing the mean of each experiment. n = 209; 208; 231 total cells. N = 5; 4; 5 independent experiments. (b) Same as A but for Muc1 and Muc1-FKBP. n = 96; 95; 135 total cells. N = 4; 3; 3 independent experiments. (c) Same as A but for Crb3 and Crb3-FKBP in polarized MDCKII monolayers. n = 71; 101; 83 total cells. N = 3; 3; 3 independent experiments In all datasets, points are colored by experiment, and each circular point represents a single cell. Colored diamonds represent experimental means. Crossbar indicates mean of experimental means, with error bars representing SD. Two-sided Student’s t-test performed on experimental means. Source numerical data are available in source data.
