Extended Data Fig. 2: Characterizing signal-recorder gene circuits.
From: Recording morphogen signals reveals mechanisms underlying gastruloid symmetry breaking
(A) Recording efficiency in Wnt-Recorder cells depends on doxycycline concentration (2i + LIF medium, 24 h recording window). (B) Recording efficiency in Wnt-Recorder cells depends on the concentration of recombinant Wnt3a ligand (200 ng ml−1 doxycycline, 24 h recording window). Shaded regions in (A-B) indicate mean ± standard deviation (3 biological replicates per condition). (C) Flow cytometry histograms corresponding to recorder-ligand crosstalk measurement in Fig. 2e. Recording windows were 6 h for Wnt Recorder and Nodal Recorder, and 3 h for BMP recorder. All ligand concentrations (including dox) were 200 ng ml−1. (D) BMP-Recorder labelling is sensitive to BMP inhibition with LDN-193189.
