Fig. 7: Non-lysosomal mTORC1 is regulated by distinct exogenous AAs.
From: Spatial and functional separation of mTORC1 signalling in response to different amino acid sources
a,b, Immunoblots with lysates from HEK293FT WT cells treated for 1 h with media containing or lacking the AA groups as shown and probed with the indicated antibodies (a) and quantification of mTORC1 activity (p-S6KT389/S6K) (b). n = 4 independent experiments. c,d, As in a and b but for RagA/B KO HEK293FT cells (c) and quantification of mTORC1 activity (p-S6KT389/S6K) (d). n = 5 independent experiments. e,f, As in c and d but for RagA/B KO HEK293FTs treated with media lacking the indicated groups or individual AAs for 1 h (e) and quantification of mTORC1 activity (p-S6KT389/S6K) (f). Data in graphs shown as mean ± s.e.m. **P < 0.01, ***P < 0.001, ****P < 0.0001. Source numerical data and unprocessed blots are available in Source data.
