Extended Data Fig. 1: Hypoxia promotes glycerol 3-phosphate biosynthesis.
a, The relative abundance of metabolites of glycolysis in HeLa cells under normoxia, hypoxia, or antimycin A (AntA, 2 μM) treatment for 8 h. b, The relative abundance of glycerol 3-phosphate and its precursor DHAP in HeLa cells under normoxia, hypoxia, or AntA (2 μM) treatment for 8 h. c, Isotopomer tracing analysis of glycerol 3-phosphate biosynthesis in HeLa cells cultured with 13C6-glucose (25 mM) or 13C5-glutamine (2 mM) for 8 h. d,e, Using N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) to modify the hydroxyl groups of glycerol and glycerol 3-phosphate and detect them by gas chromatography-mass spectrometry (GC-MS). f, Isotopomer tracing analysis of excreted glycerol in HeLa cells cultured with 13C6-glucose (25 mM) under normoxia or hypoxia for 8 h. g,h, The levels of excreted glycerol determined by GC-MS or a liquid sample glycerol assay kit in HeLa cells cultured under normoxia and hypoxia for 24 h. Values are shown as mean ± SD, n = 3 biologically independent samples, two-tailed Student’s t-tests.
