Extended Data Fig. 6: Characterization of H3K27ac in Jarid2 KO morula embryos.

a. Pie charts showing the ratios of H3K27me2-lost (C1) and intermediate (C2) putative enhancers belonging to each group of H3K27me2 dynamics. b. Scatter-plot showing the correlation between biological duplicates. Spearman correlation is also shown. c. Heatmap showing the enrichment of allelic H3K27me3 in wild-type morula embryos and allelic H3K27ac in control and Jarid2 KO morula embryos at H3K27me2-lost (C1) putative enhancers. Loci that did not have enough SNP-containing reads ( < 20) were removed from the analysis. Averaged signal profiles at the right indicate H3K27ac levels at H3K27me2-lost putative enhancers that are devoid of H3K27me3. d. Box plots showing the expression levels (log₂TPM) of the nearest genes from the putative enhancers of each cluster. The gene numbers (n) are indicated at the bottom. The centre lines in the boxes represent median values. The box edges, upper, and lower whiskers indicate the interquartile range (IQR; from the 25th to 75th percentile), the largest value smaller than 1.5× the IQR above the 75th percentile, and the smallest value larger than 1.5× the IQR below the 25th percentile, respectively. p, two-sided Mann–Whitney U-test.