Extended Data Fig. 8: Gating strategy for flow cytometry.
From: Phase separation of initiation hubs on cargo is a trigger switch for selective autophagy
Cell populations were gated through a series of steps to ensure data specificity and accuracy. First, live cells were selected using FlowJo software by plotting side scatter area (SSC-A) against forward scatter area (FSC-A), which allowed for the selection of a homogenous population based on cell granularity and size. Single cells were then gated by plotting forward scatter height (FSC-H) against forward scatter width (FSC-W) to eliminate doublets and aggregates. After gating, the data were exported as scale values and analysed using an in-house Python library mKeima (https://pypi.org/project/mkeima). GFP-positive cells were selected based on their signal in the B545-H channel. The analysis was then continued using the custom mKeima package.
