Extended Data Fig. 5: Actin is not required for NER scaling.
From: Cytoplasmic flow is a cell size sensor that scales anaphase
a, Time-lapse of a dividing cell (4-cell stage). Utrophin-GFP, actin. Blue dashed box, chromosome localization visualized by WGA-640. Blue arrow shows the orientation of chromosome separation. Insets, magnifications of the regions in the dashed boxes. Bars, 20 µm. At anaphase onset actin localizes at the cell cortex and in the cytoplasm. Cytoplasmic localization decreases during anaphase while the cortex localization remains. b, Time-lapse of a dividing cell (4-cell stage) treated with latrunculin B. Already at anaphase onset there is a very reduced cytoplasmic accumulation of actin. In (a) and (b), dashed grey line, cell boundary. t = 0 s, anaphase onset. Insets, magnifications of the regions in the dashed boxes. Bars, 20 µm. c and d, Chromosome velocity in control and latrunculin B treated embryos in a big (4-cell stage) and small cell (16-cell stage). Control, 4-cell, \({v}_{A}\) (0–100 s) n = 26 cells from 26 embryos and \({v}_{B}\) (100–200 s) n = 34 cells from 34 embryos. Latrunculin B, 4-cell, \({v}_{A}\) (0–100 s), n = 5 cells from 5 embryos and \({v}_{B}\) (100–200 s) n = 8 cells from 8 embryos. Control, 16-cell, \({v}_{A}\) (0–100 s), n = 17 cells from 16 embryos and \({v}_{B}\) (100–200 s), n = 20 cells from 19 embryos. Latrunculin B, 16-cell, \({v}_{A}\) (0–100 s), n = 5 cells from 5 embryos and \({v}_{B}\) (100–200 s), n = 8 cells from 8 embryos. e and f, Position of WGA and NER in control and latrunculin B treated embryos in a big (4-cell stage) and small cell (16-cell stage). For (e), n = 17, 9, 4 and 10 cells, from 17, 9, 3 and 10 embryos, for control and latrunculin B at 4-cell and control and latrunculin B at 16-cell stages, respectively. For (f), n = 17, 9, 3 and 9 cells, from 17, 9, 3 and 9 embryos, for control and latrunculin B at 4-cell and control and latrunculin at 16-cell stages, respectively. Statistics, two-tailed unpaired t-test. Error bars, s.e.m.
