Extended Data Fig. 1: Systematic analysis of cell size during the cleavage stage of zebrafish embryos.
From: Cytoplasmic flow is a cell size sensor that scales anaphase
a, Time-lapse of an embryo during the first 9 cleavage divisions of zebrafish development showing LifeAct-mCherry. Bars, 200 µm. b and c, Cell length (distance along the axis of chromosome separation) and cell width (distance perpendicular to the axis of chromosome separation) as a function of cell stage, respectively. Error bars, standard deviation. Center, mean. Cell length, n = 2, 12, 17, 15, 9, 16, 27, 43, 37 and 26 cells from 2, 12, 15, 9, 4, 9, 10, 10, 9 and 7 embryos; cell width, n = 2, 14, 18, 17, 7, 12, 25, 43, 28 and 24 cells from 2, 12, 15, 10, 4, 9, 10, 10, 9 and 7 embryos at 1-, 2-, 4-, 8-, 16-, 32-, 64-, 128-, 256- and 512-cell stages. d, Duration of interphase (time between consecutive mitoses) for control and dinaciclib treated embryos from 4- to 32-cell stage. Numbers indicate cell stage. Control, n = 4 cells from 4 embryos at 4-, 8-, 16- and 32-cell stages. Dinaciclib, n = 10, 12, 10, 7 cells from 10, 12, 10, 7 embryos at 4-, 8-, 16- and 32-cell stages. e and f, Time of WGA and NER in 4-cell stage control (n = 11) and dinaciclib treated (n = 6) embryos. ns = not significant. Statistics, two-tailed Mann-Whitney test. Error bars, s.e.m.
