Extended Data Fig. 3: Chromosomes and other objects in the cytoplasm move with the flows.
From: Cytoplasmic flow is a cell size sensor that scales anaphase
a, Cell in metaphase. Mitochondria, Mito-GFP. Bar, 20 µm. Yellow box, aster/spindle region. Bar, 10 µm. Blue box, cytoplasmic region. Mitochondria are homogenous in size and distribution. Bar, 5 µm. b and c, Same cell as in (a) during anaphase. Bars, 20 µm. d, Representative density profiles of Mito-GFP from ROI (grey shaded line in a, b). e-h, Time projection (e and f; 100–200 s after anaphase onset) and overlayed PIV (g and h) for mitochondria (e and g) and endogenous lipid droplets (f and h). Yellow arrow (f), droplet analysed in (i). (i) Representative velocity of the droplet highlighted in (f) and local flow around it (mitochondria PIV) as a function of time. t = 0 s, anaphase onset. j-m, Correlation of velocity (j and l) and angle difference in their trajectories (k and m) between flow velocity (mitochondria PIV) and endogenous (j and k) or injected lipid droplets (l and m), at each time point in 100–200 s after anaphase onset (n = 15 droplets x 10 time points, from 5 embryos). n and o, Time projection (100–200 s after anaphase onset) of mitochondria and chromosomes, respectively. Blue arrow, chromosomes analysed in (r). p, Merge of (n) and (o). Note similar movement of chromosomes and flows. q, PIV analysis of mitochondria. Background image, same as in (n). Red shaded area, chromosomes. Bars, 20 µm. r, Representative velocity of chromosomes highlighted in (o) and the local flow around it as a function of time. t = 0 s, anaphase onset. Note that, after 100 s (when flows are stronger), chromosome velocity and local flow are correlated. s, Angle difference between chromosomes and flow around them. Same dataset as in Fig. 2e. t, Angle difference between chromosomes and droplets around them (within ~5 µm distance). u, Time projection of endogenous lipid droplets and chromosomes. Dashed white box, one droplet is next to chromosomes. Zoomed in inset, detail of chromosomes and droplet following parallel trajectories. Bar, 50 µm. v, Trajectory of chromosomes and droplet of the cell in (u).
