Extended Data Fig. 4: Cells without DNA go through several rounds of mitosis.
From: Cytoplasmic flow is a cell size sensor that scales anaphase
a, Snapshots of an embryo (16-cell stage) with two cells dividing showing α-tubulin-ATTO-565. Grey shadowed cell, with DNA and a mitotic spindle. Blue shadowed cell (‘cell 1’), without DNA and no mitotic spindle. Bars, 50 µm. Insets, magnification of the spindle region showing H1-488 and Mito-GFP. Top cell, gey square, with DNA and H1-488 signal. Bottom cell, blue square, without DNA and no H1-488 signal. Bars, 20 µm. b, Same embryo as in (a) one cell division after (32-cell stage) showing α-tubulin-ATTO-565. The initial cell in (a) (‘cell 1’, boundaries are marked with black line) divided and generated two daughter cells (‘cell 1.1’ and ‘cell 1.2’). Bars, 50 µm. Insets, magnification of the spindle region of cell 1.1 and cell 1.2, showing Mito-GFP. Bars, 20 µm.
