Extended Data Fig. 3: NMIIB is necessary for blebbisome formation.
From: Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties
a, Quantification of blebbisomes produced by MDA-MB-231 cells after siRNA knockdown of CHMP2A, CHMP4B and NMIIB. The mean ratio of blebbisomes/cell, siControl: 0.01 + /− 0.0, siCHMP2A: 0.001 + /- 0.00, siCHMP4B: 0.01 + /- 0.00, and siNMIIB 0 + /- 0.00. n = 3 independent experiments. b, Immunoblots of NMIIB and beta Actin in MDA-MB-231 cells following NMIIB siRNA knockdown. c, Immunoblots of CHMP2A, CHMP4B and beta Actin in MDA-MB-231 cells following CHMP2A and CHMP4B siRNA knockdown. Data are from three independent siRNA knockdown experiments. P values displayed in graphs were derived from a two-tailed student’s t-test. Source numerical data are unprocessed western blots are available in source data.
