Extended Data Fig. 4: Purified blebbisomes and immunoblots of select proteins.
From: Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties
a, DIC image of MDA-MB-231 blebbisomes purified as outlined in Fig. 2a. Enlarged inset is display on the right. b, Replicative immunoblot experiments of MDA-MB-231 cells, purified blebbisomes (bleb), large Evs (lEV) and small Evs (sEV) for select proteins. Related to Fig. 2c. c, Immunoblot analysis of MDA-MB-231 (breast cancer), Gli36 (glioblastoma) and B16-F1 (melanoma) cells, blebbisomes (bleb), large Evs (lEV) and small Evs (sEV) for select proteins. Images are representative of three independent experiments. d, Summary of common protein expression pattern for MDA-MB-231, B16-F1 and Gli36 cells, purified blebbisomes, lEVs and sEVs based validation by immunoblot analysis. Based on data from Fig. 2c, d and Extended Data Fig. 3b, c. TGN38 is Trans Golgi Network Protein 2. Source numerical data and unprocessed blots are available in source data.
