Extended Data Fig. 5: Further comparison between blebbisomes and large oncosomes.
From: Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties
a, Proteomics comparison of proteins known to be enriched in large oncosomes (MYH9, MYH10, KRT18, and APEX1) as well as CD63 to serve as a known EV marker. MYH9 (p = 0.002), blebbisome mean: 196.00 + /- 4.58 SEM, large EV mean: 239.67 + /- 3.84 SEM. MYH10, blebbisome mean:18.33 + /- 2.60 SEM, large EV mean: 22.67 + /- 2.40 SEM. CD63, blebbisome mean: 3.33 + /− 0.88 SEM, large EV mean: 4.33 + /- 1.20 SEM. KRT18 (p-value = 0.007), blebbisome mean: 37.00 + /- 1.73 SEM, large EV mean: 25.67 + /- 1.33 SEM. APEX1, blebbisome mean: 0.00, large EV mean:1.33 + /- 0.88 SEM. n = 3 independent isolation preps. b, Immunofluorescence comparison based on the integrated density of maximum intensity projections of the blebbisome and large oncosome. The proteins chosen are known to be enriched in large oncosomes (MYH9, MYH10, KRT18, and APEX1) as well as CD63 to serve as a known EV marker. MYH9 (p-value < 0.0001), Blebbisome mean: 109857.81 + /- 14895.17 SEM, Large oncosome mean: 22157.01 + /- 6882.76 SEM. MYH10 (p-value = 0.0001), Blebbisome mean: 11796.96 + /- 2742.26 SEM, Large oncosome mean: 6285.41 + /- 1027.22 SEM. CD63 (p-value = 0.0066), Blebbisome mean: 5904.93 + /- 1592.75 SEM, Large oncosome mean: 2730.8 + /- 445.56 SEM. KRT18, Blebbisome mean: 4145.94 + /- 516.41 SEM, Large oncosome mean: 1943.83 + /- 105.23 SEM. APEX1 (p-value < 0.0001), Blebbisome mean: 11969.16 + /- 4898.36 SEM, Large oncosome mean: 4703.29 + /- 1537.73 SEM. n = 3 independent isolation preps. c, Maximum intensity projections of blebbisomes and large oncosomes stained for actin (magenta) and a protein of interest (green). P-values displayed in graph were determined using a two tailed student’s t-test. Source numerical data are available in source data.
