Extended Data Fig. 8: Blebbisomes contain inhibitory immune checkpoint ligands.
From: Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties
a, Immunoblot analysis of human breast cancer cells (MCF-7, T-47D, SK-BR-3, MDA-MB-231) and pro-monocytic, human histiocytic lymphoma cells (U-937) for inhibitory immune checkpoint proteins. b, Immunoblot analysis of Gli36 and B16 cells, blebbisomes (bleb), large EVs (lEV) and small EVs (sEV) for inhibitory immune checkpoint proteins. c, Replicative immunoblot experiments of MDA-MB-231 cells, purified blebbisomes (bleb), large EVs (lEV) and small EVs (sEV) for immune checkpoint proteins. Related to Fig. 6d, e. d, Immunoblot analysis of control media (Con) and EVs secreted from purified MDA-MB-231 blebbisomes for B7-H3 and PVR from three independent experiments. Rep, replicate. e, Quantification of relative signal intensity from (d). Each data point represents one independent immunoblot experiment (replicate immunoblots). n = 3 and data are displayed as mean ± s.e.m. Source numerical data and unprocessed blots are available in source data.
