Extended Data Fig. 7: Over-stretched NPCs in the Nup133-/- neural progenitor cells.
From: Nuclear pores safeguard the integrity of the nuclear envelope
(a) Summary of the over-stretched NPCs observed in the Nup133-/- neural progenitor dataset. Each example of an over-stretched NPC (#1–19) includes a representative slice through the tomograms (left), segmented membranes seen from the same orientation as in the left panel (middle), and segmented membranes seen from the cytoplasmic side (right). Based on the results of the TM analysis, the CR (yellow), IR (blue) and NR (pink) maps are projected back onto the segmented membranes (middle and right panels). The CRs clearly detached from the outer nuclear membrane are highlighted with grey arrowheads. C and N in the tomographic slices indicate the cytoplasm and nucleus, respectively. The example shown in Fig. 6b corresponds to #8. (b) Summary of large nuclear envelope openings observed in the Nup133-/- neural progenitor dataset, shown in the similar manner as in (a). Note that neither the CR, IR nor NR protomer was detected in these membrane openings by the TM analysis. Scale bars, 100 nm.
