Extended Data Fig. 6: Velocity-based ordering pinpoints detachment during XCI.
(a) Distribution of cells from Vitamin C differentiation days across latent time defined in Fig. 6b. (b) Same UMAP as in (a), based on Dam-LMNB1 levels in 100-Kb-bins of the Xi, with streamlines representing chromatin velocities of randomly coupled Dam-LMNB1 and LMNB1 readouts per cell. (c) Single-cell heatmap, showing log-transformed LMNB1 observed over expected (OE) values on the entire inactivating X-chromosome, with cells ordered along the inferred latent time shown in (b). (d) Average Dam-LMNB1 (black) and LMNB1 (grey) OE levels of the inactive X chromosome along latent time. Gap marks the moment of highest genome–lamina dynamics on the Xi. (e) Example Hoechst profile of 380 sorted single cells from the Dam-LMNB1/LMNB1 Vitamin C experiment, showing their DNA content and respective cell-cycle stage. (f) Same scatter-plot as in Fig. 6g with raw cell cycle-phase annotations determined based on Hoechst values. Cell-cycle data is available in Supplementary Table 3. Source numerical data is available in source data.
