Fig. 5: Detachment initiates before spreading of Polycomb, but follows Xist expression.
a, Same UMAP as in Fig. 4b, based on H3K27me3 levels on autosomal genes, coloured by Monocle3-inferred pseudotime. b, Average OE H3K27me3 (top) and Dam–LMNB1 (bottom) levels (y axis) across pseudotime (x axis) for the inactive X. Cells are coloured according to differentiation day. c, CF of Dam–LMNB1, H3K27me3 and their overlap for groups of 100 cells progressively slid along pseudotime (y axis) for a dynamic region of the inactive X (right) and the active (left). d, Single-cell heatmaps (n = 1,656) for H3K27me3 (left) and Dam–LMNB1 (right) for the entire inactive X. Cells (y axis) are ordered along pseudotime. The colour scale indicates log-transformed OE values for each readout. e, Scatter-plot of the average inactive X (Xi) Dam–LMNB1 (x axis) and H3K27me3 (y axis) OE levels. Cells are coloured according to differentiation day. Distributions above and on the right show average density per day. Arrow is manually drawn based on directionality of cells over differentiation days. f, Same as e, but with the inverse readout: Dam–scFc-H3K27me3 (y axis) / LMNB1 (x axis). g, Same as e, for Dam–LMNB1 (x axis) / H2AK119Ub (y axis). h, Same as e, for Dam–LMNB1 (x axis)/Xist expression (y axis). Xist levels are represented as transcripts per million. i, Summary of the order of events profiled during vitamin C differentiation, with each plotted as average OE values per differentiation day. 95% confidence interval is shown in the shaded area. Source numerical data are available in source data.
