Extended Data Fig. 6: REM transcription factors-initiated siRNA biogenesis is independent of H3K9 methylation. | Nature Cell Biology

Extended Data Fig. 6: REM transcription factors-initiated siRNA biogenesis is independent of H3K9 methylation.

From: REM transcription factors and GDE1 shape the DNA methylation landscape through the recruitment of RNA polymerase IV transcription complexes

Extended Data Fig. 6: REM transcription factors-initiated siRNA biogenesis is independent of H3K9 methylation.

a, Venn diagrams showing the relationships between reduced 24nt-siRNA clusters in suvh4 suvh5 suvh6 triple and the clsy1 clsy2 double mutants. b, Venn diagrams showing the relationships between reduced 24nt-siRNA clusters in suvh4 suvh5 suvh6 triple, clsy3 clsy4 double and the rem46 val rem12 triple mutants. c, Violin plot showing the 24nt-siRNA levels at siren loci (n = 133) in the indicated genotypes. p values calculated by pairwise t-tests are indicated. The line in the center of each violin plot represents the median. The thick black bar in the center represents the interquartile range. The whiskers represent the rest of the distribution. d, Pie charts showing the proportions of 24nt-siRNAs from all Pol IV-dependent clusters in ovule tissue that are reduced in clsy1 clsy2 or clsy3 clsy4 double mutants and the proportions of REM46 VAL REM12-dependent loci in GDE-dependent loci, GDE1-dependent siRNA among CLSY3 CLSY4-dependent group or SUVH456-dependent siRNA among CLSY1 CLSY2-dependent group. Source numerical data are available in source data.

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