Extended Data Fig. 2: GDE1 co-localizes and interacts with Pol IV complex for siRNA production. | Nature Cell Biology

Extended Data Fig. 2: GDE1 co-localizes and interacts with Pol IV complex for siRNA production.

From: REM transcription factors and GDE1 shape the DNA methylation landscape through the recruitment of RNA polymerase IV transcription complexes

Extended Data Fig. 2: GDE1 co-localizes and interacts with Pol IV complex for siRNA production.

a, Screenshot of CLSY3-9myc, CLSY3-9myc gde1-1, CLSY4-9myc, CLSY4-9myc gde1-1, Pol IV-9myc, Pol IV-9myc gde1-1 and GDE1-3FLAG ChIP-seq signals with control ChIP-seq signals subtracted over a Group 1 (CLSY3 CLSY4-dependent, GDE1-dependent) representative locus (left) or three Group 2 (CLSY3 CLSY4-dependent, GDE1-upregulated) representative loci (right). Square brackets indicate the range on a bar graph. b, Western blot showing a Co-IP assay in GDE1-3FLAG F2 crossed lines with CLSY3-9myc (left), Pol IV-9myc (middle) or VDD-9myc (right), respectively. Similar results were observed in two biological replicates. Source numerical data and unprocessed blots are available in source data.

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