Extended Data Fig. 6: Comparison of substrate binding sites in Neo1 and Dnf1. | Nature Cell Biology

Extended Data Fig. 6: Comparison of substrate binding sites in Neo1 and Dnf1.

From: P4-ATPases control phosphoinositide membrane asymmetry and neomycin resistance

Extended Data Fig. 6: Comparison of substrate binding sites in Neo1 and Dnf1.The alt text for this image may have been generated using AI.

Substrate entry site is open in the E2P state of Dnf1 in detergents (PDB ID 7KYC) (a), but is closed in the peptidisc-reconstituted Neo1 in E2P state (b, this study), and in the E2P state of Neo1 in detergents (PDB ID 7RD6) (c). Residues surrounding the substrate entry site are shown in sticks. The entry site is more tightly closed in detergent-reconstituted Neo1, involving additional residues T979, V224 and P225.

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