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Lipid trafficking

Phosphoinositide flipping governs neomycin sensitivity

Nature Cell Biology volume 27, pages 1057–1058 (2025)Cite this article

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Neomycin toxicity in eukaryotic cells stems from its binding to phosphoinositides, primarily confined to the inner leaflet of the plasma membrane. New research reveals a complex lipid-trafficking pathway that exposes phosphatidylinositol 4-phosphate on the cell surface and identifies Neo1 as a flippase that limits this exposure.

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Fig. 1: The itinerary of PI4P to the exofacial leaflet of the plasma membrane.

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Authors and Affiliations

  1. Department of Pathology, Dalhousie University, Halifax, Nova Scotia, Canada

    Michael Salsaa & Gregory D. Fairn

  2. Department of Biochemistry & Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada

    Michael Salsaa & Gregory D. Fairn

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  1. Michael Salsaa
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  2. Gregory D. Fairn
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Correspondence to Gregory D. Fairn.

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The authors declare no competing interests.

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Salsaa, M., Fairn, G.D. Phosphoinositide flipping governs neomycin sensitivity. Nat Cell Biol 27, 1057–1058 (2025). https://doi.org/10.1038/s41556-025-01707-9

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