Extended Data Fig. 3: Lipid composition of human HSPCs and progenitors.

a, Experimental design and gating to characterize the lipidome of human HSPCs and progenitors. The input material per sample, number and type of detected lipids are indicated. b, Example of extracted ion chromatogram (EIC) peak. c, Lipidomics PCA of HSPCs and progenitors isolated under different freezing conditions and from various sources as quality control. Conditions included samples with and without prior freezing; sources included commercially available samples (Stem Cell Technology; SCT) or hospital-derived samples. The analysis was based on significantly variable lipids. n = 9. d, Heatmap of all detected lipids passing quality thresholds in human untargeted lipidomics of HSPCs and progenitors. The log2FC values between paired HSPCs and progenitors per individual are depicted. n = 14. ND, not detected. e, Barplots of the summed signal intensity of lipids per class in HSPCs and progenitors. Subclasses were based on unsaturation levels [saturated (0 unsaturation), monounsaturated (1 unsaturation) and polyunsaturated (≥2 unsaturations)] or acyl-chain length [short (<36 carbons) and long (≥36 carbons)]. Significantly different abundances per class and subclass are shown. Paired two-sided Student’s t test or Wilcoxon test. NS, not significant; AUC, area under the curve. (c) n indicates the number of biological replicates per condition. For (c), 5 independent experiments were performed. For (d, e), 8 independent experiments were performed.